Become a qualified DOT oral fluid collector under 49 CFR §40.35 — the newest authorized DOT collection methodology. Study the full knowledge curriculum free and at your own pace, then complete a scheduled live Zoom proficiency demonstration with a qualified evaluator to earn your audit-ready DOT qualification.
Oral fluid became an authorized DOT collection methodology in 2023. Important: an employer cannot implement oral fluid testing until there are two HHS-certified oral fluid laboratories — one for the primary specimen and a different one for the split. As of the May 11, 2026 final rule, DOT states there are no HHS-certified oral fluid laboratories (91 FR 25508), so DOT oral fluid collections cannot lawfully be performed today. Once HHS publishes notice of the second certified laboratory, After HHS announces the second certified laboratory, §40.67 provides an 18-month transition period for certain directly observed collections — it lets employers keep conducting directly observed urine collections while they establish oral fluid capability. It is not a general 18-month delay before oral fluid applies: an employer that becomes ready to use oral fluid during that period must do so in the applicable circumstances (§40.67(g)(6)). ODAPC will publish a Federal Register notice when the period begins and ends. This qualification prepares you to collect the day oral fluid becomes available to your employer. This qualification is built to the collector training requirements of 49 CFR §40.35, so you can perform DOT oral fluid specimen collections across the federally regulated transportation programs — FMCSA, FAA, FTA, FRA and PHMSA, plus the Coast Guard — adding a fast, directly-observed option to your collection site, clinic, C/TPA, or in-house program.
Work through the full §40.35 knowledge curriculum online at your own pace. No signup or payment to start.
Schedule a live session and perform your five supervised error-free mock collections, monitored by a qualified evaluator.
Receive dated, evaluator-signed DOT qualification documentation — audit-ready for an inspector.
Authorized DOT methodology since 2023 (49 CFR §40.35)
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Yes. DOT authorized oral fluid testing in a 2023 final rule, and 49 CFR §40.35 sets the collector training requirements. It joins urine as an authorized DOT specimen type. However, an employer cannot implement it until two HHS-certified oral fluid laboratories exist — one to test the primary specimen and a different one to test the split. In its May 11, 2026 final rule DOT stated that, as of publication, there are no HHS-certified oral fluid laboratories (91 FR 25508), so collections cannot lawfully be performed today. After HHS notices the second lab, After HHS announces the second certified laboratory, §40.67 provides an 18-month transition period for certain directly observed collections — it lets employers keep conducting directly observed urine collections while they establish oral fluid capability. It is not a general 18-month delay before oral fluid applies: an employer that becomes ready to use oral fluid during that period must do so in the applicable circumstances (§40.67(g)(6)). ODAPC will publish a Federal Register notice when the period begins and ends. Qualifying now positions you for when it becomes available.
Yes. The curriculum and proficiency demonstration are built to 49 CFR §40.35 — the qualification training plus the five consecutive error-free mock collections DOT requires before you may perform collections.
Yes. The knowledge modules are free to work through with no signup. Your one-time $150 covers the part that actually qualifies you: the scheduled live Zoom proficiency demonstration with a qualified evaluator, plus your audit-ready qualification packet.
Yes. §40.35(b)(2) requires training to proficiency on the particular device(s) you will use, and §40.35(c) requires five consecutive error-free mock collections for each device. Procedure steps come from the ODAPC “DOT Oral Fluid Specimen Collection Procedures Guidelines” and the device manufacturer’s instructions. (The OFMG is a separate thing — the HHS Oral Fluid Mandatory Guidelines, which govern oral fluid testing. Be precise about what is certified: HHS certifies laboratories. A collection device is not separately “HHS-certified” in its own right — a listed device is approved for use by a particular HHS-certified laboratory through the NLCP process.)
DOT requires refresher qualification training at least every five years, and error-correction training if a mistake causes a test to be cancelled. Your lifetime access lets you refresh the material anytime.